Blue Fluorescent Protein Vector

Vector blue is both chromogenic and fluorescent.

Blue fluorescent protein vector. Bright blue fluorescence. Roger tsiens lab contains the insert blue fluorescent protein and is published in trends biochem sci. This plasmid is available through addgene. Extremely high ph stability.

Through dual laser excitation of bright and photoinduced dark states mutations to the residues surrounding the bfp chromophore enable long wavelength optical modulation of. The prsetcfp cyan and prsetbfp blue vectors contain alternative color versions of fluorescent proteins derived from aequorea victoria. Monomeric fast maturing protein with successful performance in fusions. Recommended as a donor for green fluorescent proteins in fret applications.

Fluorescent proteins are genetically encoded tools that are used extensively by life scientists. Ideally it can be combined with your living color dsred polyclonal raised in rabbit cat. Blue fluorescent protein bfp fusion protein vectors. Which additional fluorescent protein and which of your abs would you recommend.

Tagbfp monomeric blue fluorescent protein. The green fluorescent protein gfp is a protein composed of 238 amino acid residues 269 kda that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. These proteins are commonly used with the yfp and emgfp for multi color applications. Commonly used fluorescent protein genes and vectors.

However non xylene clearing agents and non xylene based mounting media such as vectamount h 5000 must be used because the reaction product is soluble in xylene. Blue fluorescent proteins bfps offer visualization of protein location and behavior but often suffer from high autofluorescent background and poor signal discrimination. These proteins are commonly used with the yfp and emgfp for multi color applications. The original green fluorescent protein gfp was cloned in 1992 prasher et al gene 1992 and since then scientists have engineered numerous gfp variants and non gfp proteins that result in a diverse set of colorsaddgene has assembled a collection of empty plasmid backbones with different.

These combined dna sequence and map files can be opened with snapgene or the free snapgene viewer. Similar proteins that also fluoresce green are found in many marine organisms but the label gfp traditionally refers to this particular protein which was first isolated from the jellyfish aequorea. Sections stained with the vector blue substrate can be dehydrated cleared and permanently mounted. Updated december 16th 2019.

Fluorescent protein genes plasmids. Fluorescent protein genes plasmidszip 4 mb 385 sequences. 632496 and an anti gfp raised in chicken which we are currently using to stain for our constructs containing the dsred and gfp protein.