Mbp His Tag Vector

To clone into this vector add lic fusion tags to the 5 end of your pcr primers.

Mbp his tag vector. 2 304end with n terminal his tag mw34 kda expressed in an e. This plasmid is an empty vector to be used with a lic cloning protocol. The pmal c6t vector is designed to produce maltose binding protein mbp fusions in the cytoplasm. Mbp can enhance your proteins solubility and expression.

C terminal polyhistidine 6x his tag is encoded by the vector for optional addition to the target protein. Maltose binding protein from e. The vector expresses an n terminal his tagged male gene followed by a multiple cloning site containing a tev protease recognition sequence allowing mbp to be cleaved from the protein of interest after purification. The vector pmal c5x his is designed to produce maltose binding protein mbp fusions where the protein of interest can be cleaved from mbp with the specific protease factor xa neb p8010.

The cytoplasmic his6mbp destination vector pdest hismbp was constructed by inserting an in frame hexahistidine sequence between codons three and four of the open reading frame orf encoding mbp in pkm596 as detailed elsewhere tropea et al. Human myelin basic protein mbp genbank accession no. A variant vector that will produce a cleavable n terminal mbp and cleavable c terminal his tag was made by inserting dna encoding mbp and a tvmv protease recognition sequence in front of the lic site of pmcsg28. It can also be used as an affinity tag.

We provide vectors for the expression of target proteins with either an n terminal mbp tag or an n terminal 6xhis mbp tag pab 6xhis mbp. Michael j fox foundation mjffs lab contains the insert mbp maltose binding protein and is published in unpublished this plasmid is available through addgene. It has a tev cleavable his6 fusion tag on its n terminus. The periplasmic his mbp expression vector pdest peri hismbp was assembled as follows.

Coli cell expression system. Bacterial mbp tag vector set enables you to compare placing maltose binding protein mbp solubilityaffinity tags at either the n or c terminus of your gene of interest inserted into the mcs under transcriptional control of the oxb20 strong bacterial promoter with and also without a tev tobacco etch virus protease cleavage site. The mbp tag is sometimes combined with a 6xhis tag as the latter is more widely used for affinity purification.