Ptre Tight Vector

To see this sequence with restriction sites features and translations please download snapgene or the free snapgene viewer. Molecularcloud plasmid repository offers ptre tight mir 1 akaptre m1d for ptre tight mir 1 akaptre m1d plasmid repository dna construct gene clone purpose. Roberta gottliebs lab contains the insert mitotimer and is published in autophagy.

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources.

Ptre tight vector. Article snippetthe ptre enhanced green fluorescent protein egfp vector was generated by inserting an egfp gene clontech into the multiple cloning site of the ptre tight vectorclontech. Vector map and multiple cloning site mcs of ptre tight acgfp1 vector. Welcome to vector database. Plasmid ptre tight mitotimer from dr.

A newer version of the response plasmid ptre tight clontech does not have the reporter gene nor a selection marker to allow for making stable mammalian cell lines. It is not intended as a cloning vector. Epub 2013 oct 11. Ptre tight bi acgfp1 is a bidirectional tre tight plasmid that can be used to inducibly express a reporter green fluorescent protein acgfp1 along with a gene of interest with our.

In the ptre tight egfp plasmid clontech the mcs has been replaced with egfp26making cloning the gene of interest very difficult. All sites shown are unique. This vector can be used as a reporter of induction efficiency using standard luciferase detection reagents. This is a free resource for the scientific community that is compiled by addgene.

Vector type ptre tight antigengene or protein targets spastin relevance plasmids for the study of hereditary spastic paraplegia. This plasmid is available through addgene. Ptre tight vector for expressing a gene with the tet on advanced or tet off advanced system. The ptre tight luc control vector packaged with the ptre tight vector contains an ad ditional 1649 bp encoding firefly luciferase inserted into the mcs.