Ta Vector Sequence

Pcr21 topo parental bacterial expression vector for topo ta cloning of pcr products. The ta cloning kit contains the ta cloning vector and all the reagents needed for ligation. T vectors pmd20 and pmd19 for pcr cloning pmd20 and pmd19 simple t vectors are linearized pcr cloning vectors cleaved by ecorv with single 3 terminal thymidine residues dt at both ends.

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources.

Ta vector sequence. M13 forward m13 reverse t7 and t3. This means sequencing reactions give less vector sequence and more insert sequence. This tailing leaves the vector with a single 3 overhanging thymine residue on each blunt end. The target vector is linearized and cut with a blunt end restriction enzyme.

This is a free resource for the scientific community that is compiled by addgene. These t overhangs at the cloning site improve the efficiency of ligation of pcr products that contain da overhangs. Quick avoid inefficient ligation and laborious searches for appropriate restriction enzymes. Quick and efficient pcr cloning with topo ta cloning clone pcr amplified dna fragments blunt or a overhang directly into a choice of over 40 subcloning sequencing or expression vectors in just 5 minutesand obtain up to 95 recombinant clones.

Home resources plasmid files ta and gc cloning vectors rbc ta cloning vector linearized rbc ta cloning vector linearized linearized vector with 3 t overhangs for ta cloning of pcr products with bluewhite screening. View chapter purchase book. This page is informational only this vector is not available from addgene please contact the manufacturer for further details. The topo ta cloning vector was designed so that when linearized it has single 5 thymidine overhangs at each end which is covalently attached to the topoisomerase i enzyme.

This vector is then tailed with dideoxythymidine triphosphate ddttp using terminal transferase. Pcr4 topo ta clone selection and manipulation. It is important to use ddttp to ensure the addition of only one t residue. The kits include an aliquot of each.

When the 5 end of the insert nears the 3 t overhang the energy from the covalent bond transfers to connect the insert and vector. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by addgeneonly the plasmids deposited at addgene are available for purchase through this website. Welcome to vector database.

The pcr4 topo ta vector has sites for 4 common sequencing primers. Molecular cloning assisted by vectors is the most popular and common method to obtain genes of interest. Yeastern biotechs ta cloning kit offers a quick reliable and efficient method for cloning a variety of dna sequences.