Topo Xl Vector Sequence

Description the topo xl 2 complete pcr cloning kit provides all the necessary elements for efficient cloning of extra long pcr products up to 13 kb.

Topo xl vector sequence. Coli gene ccdb bernard et al 1994. The energy from this breakage is stored as a covalent bond between the cleaved 3 dna strand and a tyrosyl residue of topoisomerase i 1. The vector contains the b gene fused to the c terminus of the lacza fragment. Pcr xl topo linearized pcr21 topo linearized pcr4 topo linearized pcr8gwtopo linearized.

Thermo fisher invitrogen download free trial get snapgene viewer. Pcr xl topo allows direct selection of recombinants via disruption of the lethal e. Linearized mammalian vector with 3 t overhangs and bound topoisomerase for topo ta cloning of pcr products and high level expression. Pcr xl topo parental vector for topo ta cloning of long pcr products.

To see this sequence with restriction sites features and translations. This is a free resource for the scientific community that is compiled by addgene. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. Pcr4 topo parental sequencing vector for topo ta cloning of pcr products.

Similar to pcr21 topo but with an sp6 promoter. Directional topo cloning vector for creating a gateway entry vector that is suitable for recombination with a destination vector. The kit uses the linearized and topoisomerase 1 activated pcr xl 2 topo vector which is compatible with the cloning of blunt end pcr fragments. This page is informational only this vector is not available from addgene please contact the manufacturer for further details.

A topological vector space x is a vector space over a topological field most often the real or complex numbers with their standard topologies that is endowed with a topology such that vector addition x x x and scalar multiplication x x are continuous functions where the domains of these functions are endowed with product topologies. Pcr xl 2 topo linearized pcr xl topo linearized. Ligation of a long pcr product disrupts expression of the lacza ccdb gene fusion permitting growth of only. The topo technique specifically uses vaccinia virus isolated topoisomerase i as this enzyme recognizes the dna sequence 5 ctcctt 3 and digests double stranded dna at this sequence.