Bacterial Artificial Chromosome Vector Ppt

They contain ori and genes which encode ori binding proteins.

Bacterial artificial chromosome vector ppt. It must be small in size. Since they are present in low copies recombination between the high copy plasmids. The bacterial artificial chromosomes usual insert size is 150350 kbp. Dna cloning vector a plasmid of bacteria that brings the foreign dna fragment into the genome of the host cell examples.

Yeast artificial chromosomes is a human engineered dna molecule used to clone dna sequences in yeast cells. Yeast artificial chromosome yac first described in 1983 by murray and szostak. Cloning vector is used for replicating donor dna fragment within host cell. It is derived from naturally occurring f plasmid.

Bacs are often used to sequence the genome of organisms in genome projects for example the human genome project. These vectors can hold dna fragments of up to 300 kb. They are capable of carrying approximately upto 300 kbp of inserted dna sequence. These proteins are critical for bac replication.

Bacterial artificial chromosomes bacs also are used to express the full length human hd gene. Cloning vector characteristics and types cloning vector. Bacterial artificial chromosome vectors bacs were developed to permit the cloning and stable maintenance of large 100200 kb pieces of dna in e. A similar cloning vector called a pac has also been produced from the dna of p1 bacteriophage.

Their stability and ease of handling have made these vectors increasingly popular for whole genome mapping and sequencing projects from microbes plants and animals. Plasmid bacteriphage cosmid yeast artificial chromosomes. Host cell a cell that receives recombinant dna for cloning purpose host cell. Bacterial artificial chromosome a bacterial artificial chromosome bac is a dna construct based on a functional fertility plasmid or f plasmid used for transforming and cloning in bacteria usually e.

Characteristics of a cloning vectors. Yacs are shuttle vectors capable of replicating and being selected in common bacterial hosts such as ecoli as well as in the yeast s. They are capable of carrying approximately upto 1000 kbp of inserted dna sequence. Bacterial artificial chromosomes are similar to e.

It must be self replicating inside host cell. Many different yac plasmids exist such as pyac3 pyac4. Bachd mice express 97 cag repeats and exhibit progressive motor deficits beginning at approximately 2 months of age neuronal synaptic dysfunction and late onset selective neuropathology gray et al 2008. Coli f factor and so can be maintained in a single copy per cell.

Enzymes used in join targeted dna fragment with the vector to form recombinant dna.