Lentiviral Vector Purification Using Nanofiber Ion Exchange Chromatography

Tinch s szczur k swaney w reeves l witting sr. This novel scalable stationary phase provides a high surface area that is accessible to lv and therefore has potential for high capacity operation compared to traditional bead based supports. This work investigates whether nanofiber based ion exchange chromatography can provide a scalable lv purification process.
lentiviral vector purification using nanofiber ion-exchange chromatography

A direct capture method based on ion exchange chromatography and cellulose nanofibers for lv concentration and purification was developed.

Lentiviral vector purification using nanofiber ion exchange chromatography. Eds viral vectors for gene therapy. Humana press new york ny. Lentiviral vectors lvs are used in cell and gene therapies due to their ability to transduce both dividing and non dividing cells while carrying a relatively large genetic payload and providing long term gene expression via gene integration. Typically a lv is produced via transient plasmid dna pdna transfection of adherent hek293 cells in multiple t175 flasks or cell factories where 4872 h post transfection lentivirus containing mediums lcms are harvested and processed.

This novel scalable stationary phase provides a high. Lentivirus vector purification using anion exchange hplc leads to improved gene transfer article pdf available in biotechniques 3451074 8 1080 june 2003 with 1244 reads how we measure. Lentiviral vectors lvs are used in cell and gene therapies due to their ability to transduce both dividing and non dividing cells while carrying a relatively large genetic payload and providing long term gene expression via gene integration. A direct capture method based on ion exchange chromatography and cellulose nanofibers for lv concentration and purification was developed.

Manfredsson f benskey m. Large scale purification of a lentiviral vector by size exclusion chromatography or mustang q ion exchange capsule. 2019 a scalable lentiviral vector production and purification method using mustang q chromatography and tangential flow filtration. Lentiviral vector purification using nanofiber ion exchange chromatography.

Methods in molecular biology vol 1937. Ion exchange chromatography was used to purify lentiviral particles based on the fact that lentivirus are retained by the anionic exchange columns and can be eluted subsequently with buffers. Lentiviral vector purification using nanofiber ion exchange chromatography lentiviral vectors lvs are used in cell and gene therapies due to their ability to transduce both dividing and non dividing cells while carrying a relatively large genetic payload and providing long term gene expression via gene integration. First online 01.