Mammalian Expression Vector Addgene
Mammalian expression vectorszip 7 mb 347 sequences.
Mammalian expression vector addgene. Lentiviral vector for mammalian expression of doxycycline inducible cas9 and constitutive expression of blasticidin s deaminaase t2a rtta pcw cas9 50661. These combined dna sequence and map files can be opened with snapgene or the free snapgene viewer. The following crispr plasmids have been designed for use in mammalian expression systems. For mammalian cell expression pgl2 luc addgene 26280.
Updated december 16th 2019. Neomycin should not be used for mammalian expression instead use g418. This can be confusing since the neokan gene confers g418 resistance. Commonly used vectors for gene expression in mammalian cells.
Talens expressed from these vectors also have a flag epitope tag and nuclear localization signal at their amino terminus. This website uses cookies to ensure you get the best experience. All expression vectors listed below have the cmv promoter for mammalian cell expression and a t7 promoter for in vitro transcription of rna. By continuing to use this site you agree to the use of cookies.