Pet Vector Copy Number

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources.

Pet vector copy number. These combined dna sequence and map files can be opened with snapgene or the free snapgene viewer. In this system there is a t7 promoter that can be acted upon by t7 rna polymerase to drive high level expression of the gene of interest. The vector encodes two multiple cloning sites each of which is preceded by a t7 promoter lac operator and ribosome binding sites. This page is informational only this vector is not available from addgene please contact the manufacturer for further details.

For example the pmb1 ori maintains about 20 copies per cell while puc which differs by only two mutations will produce as many as 700 copies per cell. If you choose a plasmid with the ori pbr322 puc18 pbsks pet system you will have 20 30. A plasmids copy number has to do with the balance between positive and negative regulation and can be manipulated with mutations in the replicon. Find msds or sds a coa data sheets and more information.

The vector utilizes the t7lac promoter system for strong and tightly controlled gene expression. The translation vector names are distinguished from the transcription vector names by the addition of a letter suffix following the name eg pet 21a which denotes the reading frame relative to the bam h i cloning site recognition sequence ggatcc. Pet duet vectors novagen bacterial expression and tagging vectors. Petduet 1 dna novagen petduet 1 is designed for the coexpression of two target genes.

The pet vector exists as a low copy number plasmid in host e. Tags and fusion partners. Coli which reduces leaky expression before induction. Pet sumo linearized linearized bacterial vector with 3 t overhangs for ta cloning and inducible expression of an n terminally 6xhis and sumo tagged protein.

Updated december 16th 2019. The ori of your plasmid controls the number of plasmid copies in the bacteria for example in e. Welcome to vector database. Your time is valuable.