Puc18 Vector Pdf

The puc18 and puc19 plasmids enable successful cloning of large dna fragments larger than those cloned with a m13 mp18 rf phage vector.

Puc18 vector pdf. Use with snapgene software or the free viewer to visualize additional data and align other sequences. These changes together result in a temperature dependent copy number of. Puc19 is one of a series of plasmid cloning vectors created by joachim messing and co workers. Plasmid sequence and annotations.

Vector containing portions of pbr322 and m13mp19 1. Joachim messings lab is published in gene. These cloning vectors contain a multiple cloning site at the lacz region that enables recombinant plamids to be verified via bluewhite colony screening using agar plates containing iptg and x gal. The mcs is reversed in puc19.

This plasmid is available through addgene. Naturally occurring plasmids are viruses of bacteria. Expression of target dna is enabled by the presence of a. Coli vector with a multiple cloning site mcs for dna cloning.

Puc1819 vector map multiple cloning sites of puc18. The artificial plasmid puc18 has been genetically engineered to include 1 a gene for antibiotic resistance to ampicillin amp r and 2 a gene and its promoter for the enzyme beta galactosidase lacz. Vector author first author. Form of vector example plasmid 10 kb double stranded circular dna puc18 bacteriophage 20 kb linear dna l2001 cosmid 35 45 kb double stranded circular dna scos 1 yacs yeast artificial chromosomes 200 1500 kb double stranded circular dna pyac.

Use text editor or plasmid mapping software to view sequence. M13 hindlll bevl 5 g taa aac gac ggc cag tgc caa gct tgc atg cct gca ggt cga ctc tag agg atc ccc ggg tac cga gct cga att c 3 c att ttg ctg ccg gtc acg gtt cga acg tac gga cgt cca gct gag atc tcc tag ggg ccc atg gct cga gct taa g. Plasmid puc18 from dr. It con tains the pmb1 origin of replication from pbr322 but it lacks the rop gene and carries a point mutation in the rnaii transcript g 2975 in pbr322 to a 1308 in puc19.